rabbit anti sox1  (Cell Signaling Technology Inc)

Journal: Genes

Article Title: Role of Neurocellular Endoplasmic Reticulum Stress Response in Alzheimer’s Disease and Related Dementias Risk

doi: 10.3390/genes15050569

Figure Lengend Snippet: Validation of generated NSCs. ( a ) A schematic outline of the NSC differentiation method. ( b ) Representative ICC images showing expression of NSC markers Nestin, PAX6, SOX2, and SOX1 in generated NSCs. ( c ) Heap map of transcriptome-wide differentially expressed genes between iPSCs and their differentiated NSCs. ( d ) Cell type gene set (PanglaoDB Augmented 2021) enrichment analysis plot of the generated NSCs’ upregulated transcriptome. ( e ) Bar plot showing dorsal neuroepithelial gene expression profile of the generated NSCs.

Article Snippet: The ICC analysis of the NSC markers was performed using commercially available the primary antibodies mouse anti-human Nestin (MA1-110, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-human PAX6 (#60443, Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-human SOX2 (sc-365964, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and rabbit anti-human SOX1 (#4194, Cell Signaling Technology, Inc., Danvers, MA, USA); the secondary antibodies donkey anti-mouse Alexa FluorTM 488 and donkey anti-rabbit Alexa FluorTM 594 (R37114 and R37119, respectively; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA); and standard ICC techniques.

Techniques: Biomarker Discovery, Generated, Expressing, Gene Expression

Journal: Genes

Article Title: Role of Neurocellular Endoplasmic Reticulum Stress Response in Alzheimer’s Disease and Related Dementias Risk

doi: 10.3390/genes15050569

Figure Lengend Snippet: Validation of generated NSCs. ( a ) A schematic outline of the NSC differentiation method. ( b ) Representative ICC images showing expression of NSC markers Nestin, PAX6, SOX2, and SOX1 in generated NSCs. ( c ) Heap map of transcriptome-wide differentially expressed genes between iPSCs and their differentiated NSCs. ( d ) Cell type gene set (PanglaoDB Augmented 2021) enrichment analysis plot of the generated NSCs’ upregulated transcriptome. ( e ) Bar plot showing dorsal neuroepithelial gene expression profile of the generated NSCs.

Article Snippet: The ICC analysis of the NSC markers was performed using commercially available the primary antibodies mouse anti-human Nestin (MA1-110, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-human PAX6 (#60443, Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-human SOX2 (sc-365964, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and rabbit anti-human SOX1 (#4194, Cell Signaling Technology, Inc., Danvers, MA, USA); the secondary antibodies donkey anti-mouse Alexa FluorTM 488 and donkey anti-rabbit Alexa FluorTM 594 (R37114 and R37119, respectively; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA); and standard ICC techniques.

Techniques: Biomarker Discovery, Generated, Expressing, Gene Expression